Figure 3

Stimulation with autologous tumour homogenate elicits IFN-γ production in lymphocytes from sentinel node. In six of 15 patients, IFN-γ secretory responses were investigated with ELISA on culture supernatants. Tumour infiltrating lymphocytes (TIL), lymphocytes from sentinel nodes (SN1-3), from nonsentinel node (LN) and in peripheral blood leucocytes (PBL) from patient no 5, were cultured with medium alone (black bars), autologous tumour homogenate diluted 1/100 (open bars), 1/10 (grey bars) (A) or ConA 10 μg ml⁻¹ (B). The IFN-γ production was measured in pooled triplicates of supernatants collected at day 5.