Figure 4
High spontaneous proliferation and specific IFN-γ production upon addition of tumour homogenate in cells from a sentinel node in a case of Duke's C. In six of 15 patients, IFN-γ secretory responses investigated with ELISAs on culture supernatants. A single cell suspension from the sentinel node from patient no 13 was incubated with 10- and 100-fold dilutions of autologous tumour homogenate in a time course proliferation assay. Cells were pulsed with 1 μCi 3H-Thymidine, 18 h before harvesting. Error bars indicate s.e.m. (A). The corresponding IFN-γ production by the sentinel node acquired lymphocytes was measured in an ELISA on pooled triplicates of supernatant collected day 7 (B).
