Table 2 Proliferative responses in lymphocytes from sentinel node, the tumour, nonsentinel node and peripheral blood upon stimulation with autologous tumour extract

	SN				TIL				LN				PBL
Pat nr	BG ^a	Peak ^b	Peak-BG	SI ^c	BG ^a	Peak ^b	Peak-BG	SI ^c	BG ^a	Peak ^b	Peak-BG	SI ^c	BG ^a	Peak ^b	Peak-BG	SI ^c
1	9672	26 289	16 617	2,7		ND			4299	7141	2842	1,7	2496	4731	2235	1,9
2	4049	14 559	10 510	3,6	382	1485	1103	3,9	818	509	—	0,6	2836	3301	465	1,2
3	1207	15 125	13 918	12,5	196	1022	826	5,2	2201	3134	933	1,4	553	288	—	0,5
4	1104	43 446	42 342	39,4	186	546	360	2,9		ND			4570	2536	—	0,6
5		ND				ND				ND				ND
6	9810	55 430	45 620	5,7	1037	2482	1445	2,4	9495	35 032	25 537	3,7	1592	4245	2653	2,7
7	3555	32 141	28 586	9,0		ND			2155	79 629	77 474	37,0	1360	23 257	21 897	17,1
8	1051	6270	5219	6,0		ND			5400	110 655	105 255	20,5	2168	3620	1452	1,7
9	449	3283	2833	7,3		ND				ND			1092	1377	285	1,3
10	488	1218	730	2,5		ND			313	1109	796	3,5	50	52	2	1,0
11	127	238	111	1,9	262	511	249	2,0	410	3878	3468	9,5^d	645	627	—	1,0
12	341	3848	3507	11,3^d	423	1237	814	2,9	1009	3223	2214	3,2	180	797	617	4,4
13	66901	97 370	30 469	1,5		ND				ND			27 333	56 664	29331	2,1
14	3328	4506	1178	1,4		ND				ND			796	13 331	12535	16,7
15	398	248	—	0,6	106	131	25	1,2	215	131	—	0,6	120	126	6	1,1

All values are given in counts per minute (cpm) except the Stimulation Index (SI).
SN, sentinel node; TIL, tumour infiltrating lymphocytes; LN, nonsentinel lymph nodes; PBL, peripheral blood leukocytes.
ND=Not determined.
—=Below background value.
^aBG=Background, proliferation in complete medium, mean value from triplicate.
^bPeak=The highest proliferation upon stimulation with autologous tumour extract, mean value from triplicate.
^cSI=stimulation index=peak/BG.
^dSingle high measurements without dose–response or antigen dependent kinetics.

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