Figure 5
From: Prostaglandin E2 stimulates progression-related gene expression in early colorectal adenoma cells
Gene expression pattern in adenoma cells. RNA was isolated from VACO235 and LT97 adenoma cell cultures at about 50% confluency. Expression of COX-2, VEGF and c-fos was determined by RT–PCR. Expression of GAPDH was determined for standardisation. Polymerase chain reaction products were analysed on 6% acrylamide gels. The figure shows a composite of two individual analyses each with its separate GAPDH control.
