Table 1 Polymerase chain reaction conditions for assessment of gene expression
From: Prostaglandin E2 stimulates progression-related gene expression in early colorectal adenoma cells
Gene | Primer sequences | Annealing temperatures | Cycles |
|---|---|---|---|
GAPDH | |||
Sense | 5′-CGGGAAGCTTGTGATCAATGG-3′ | 55°C | 22 |
Antisense | 5′-GGCAGTGATGGCATGGACTG-3′ | ||
c-fos | |||
Sense | 5′-CCTGTCAAGAGCATCAGCAGCATGG-3′ | 60°C | 29 |
Antisense | 5′-GAGTACAGGTGACCACCGGAGTGC-3′ | ||
COX-2 | |||
Sense | 5′-TTCAAATGAGATTGTGGGAAAAT-3′ | 55°C | 32 |
Antisense | 5′-AGATCATCTCTGCCTGAGTA-3′ | ||
VEGF | |||
Sense | 5′-CTGCTGTCTTGGGTGCATTG-3′ | 53°C | 30 |
Antisense | 5′-CACCGCCTCGGCTTGTCACAT-3′ | ||
