Figure 3

Immunocytochemistry of V5-His-tagged PIF transiently transfected into HuH7 cells in the pcDNA3.1+V5His PIF vector. Following transfection, cells were treated with the inhibitor of glycoprotein secretion monensin, fixed and co-stained with an FITC-labelled anti-V5His secondary and Hoescht 33258. FITC fluorescence was not observed without monensin treatment.