Figure 2

Constitutive expression of GCP-2 in SCLC cells is mediated by NF-κB. (A) Expression of NF-κB p65 was detected by Western blotting in both cytoplasmic (C) and nuclear (N) fractions in SCLC cell lines. In contrast, phosphorylated p65 (pp65) was only detected in nuclear fractions in those cells. (B) ALLN significantly reduced GCP-2 release, measured by ELISA, in a dose-dependent fashion in H711, H345 and H69 cells. GCP-2 was reduced at least 80% when cells were treated at a final concentration of 10 and 50 μ M of ALLN in all SCLC cell lines tested. Each bar is the mean±s.e.m. of three determinations from two independent experiments. (C) Nuclear p65, detected by immunofluorescence, was reduced following treatment with ALLN at a final concentration of 10 μ M in H345 cells.