Figure 1

Immunohistochemical analysis of FGFR4, CD-31 and Ki-67 in primary melanoma tissues. (A) No expression of FGFR4 in a tumour nest (arrows) of a superficial spreading melanoma (SSM). (B) Negative control of A after preincubation of the FGFR4 antibody with a blocking peptide. (C and D) Expression of FGFR4 (red) in a nodular malignant melanoma (NMM) with high intensity (C) and in an SSM with intermediate intensity (D). (E) Expression of FGFR4 in an NMM with areas of high intensity (arrow) and intermediate intensity (arrowhead). (F) H&E stained section of E. (G) Expression of CD-31 in vessels (red) in an FGFR4-positive NMM. (H) Expression of Ki-67 (red) in proliferating melanoma cells of an FGFR4-positive SSM. Scale bar, 100 μm.