Table 3 Ad5-Δ24RGD viral replication in doxorubicin-treated OS cells as determined by quantitative PCR analysis

From: Different susceptibility of osteosarcoma cell lines and primary cells to treatment with oncolytic adenovirus and doxorubicin or cisplatin

OS cells

Genomes × 10 5 −dox a

genomes × 10 5 +dox

+dox/−dox

Average ratio: +dox/−dox (±s.d.)

 

5.8

3.5

0.6

 

OS-11

3.3

0.9

0.3

0.4 (±0.2)

 

4.1

1.1

0.3

 
 

6.5

1.4

0.2

 

OS-12A

1

0.2

0.2

0.2 (±0.04)

 

3.4

0.5

0.1

 
 

17

16

0.9

 

OS-16

8.9

14

1.6

1.3 (±0.3)

 

75

94

1.3

 
 

2.1

2.9

1.4

 

SaOs-2

5.5

6.4

1.2

1.1 (±0.3)

 

11

8

0.7

 
 

1.6

3.4

2.1

 

MG-63

7.7

7

0.9

1.2 (±0.8)

 

11

6.2

0.6

 
 

20

15

0.8

 

U2OS

96

127

1.3

1.0 (±0.3)

 

87

72

0.8

 
 

2.1

4.8

2.3

 

SaOs-2b

5.5

5.1

0.9

1.8 (±0.8)

 

11

25

2.3

 
  1. aThe number of adenovirus genomes in cells cultured in the absence (−dox) or presence (+dox) of a subtoxic dose doxorubicin was determined in three independent experiments and the average ratio and s.d. was calculated.
  2. bSaOs-2 cells treated with a toxic dose of doxorubicin.
  3. PCR=polymerase chain reaction; OS=osteosarcoma.
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