Table 1 Expression levels of mRNAs for CEA, CEACAM1, CEACAM6, CEACAM7, MUC2, MMP7, and CK20 in primary colorectal tumours compared to control colonic epithelial cells

From: Biomarker selection for detection of occult tumour cells in lymph nodes of colorectal cancer patients using real-time quantitative RT–PCR

 

Tumour

Crypt iEC a

Luminal iEC

mRNA species

Median b

IQR c

Median

IQR

P -value d

Median

IQR

P -value

CEA

107

39–1207

261

225–537

NS

393

212–769

NS

CEACAM6

20

6.5–55

28

14–35

NS

38

13–116

NS

CEACAM1-S

5.1

2.5–14

19

12–34

<0.01

28

12–72

<0.01

CEACAM1-L

1.4

0.43–6.4

16

8.0–40

<0.001

22

10–40

<0.001

CEACAM7-1

0.05

0.005–0.37

0.90

0.67–2.9

<0.001

1.9

1.2–6.0

<0.001

CEACAM7-2

9.0

0.94–32

302

162–468

<0.001

483

361–582

<0.001

MUC2

0.66

0.04–3.4

32

25–56

<0.001

33

17–77

<0.001

MMP7

1.7

0.85–4.3

0.09

0.01–0.25

<0.01

0.09

0.003–16

<0.05

CK20

14

5.5–40

295

178–675

<0.001

162

104–377

<0.001

  1. aiEC=intestinal epithelial cells.
  2. bMedian mRNA copies/18S rRNA unit of 20 CRC tumour samples (2 Dukes' Stage A, 13 Dukes' Stage B, 2 Dukes' Stage C and 3 Dukes' Stage D), 14–20 crypt iEC samples and 10–20 luminal iEC samples. Equal numbers of iEC samples were derived from the apparently healthy resection margin of colon from patients operated for CRC and from colon of UC patients. The mRNA expression levels for any of the markers in iEC from normal colon of CRC patients and UC colon, respectively, did not differ significantly from each other with the exception of MMP7 mRNA. MMP7 mRNA levels were significantly higher (P<0.01) in the iECs from UC patients compared to iEC from the resection margin of CRC patients. MMP7 values for iEC in the Table include data from 10 CRC and 10 UC patients.
  3. cIQR=interquartile range from the 25 to the 75 percentile.
  4. dP-value obtained by comparing mRNA expression levels in tumours with those in crypt iECs, and luminal iECs using Kruskal–Wallis nonparametric one-way ANOVA with Dunn's multiple comparison post hoc test. NS=not significant, that is P-value >0.05.
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