Table 2 Expression levels of mRNA for CEA, CEACAM1, CEACAM6, CEACAM7, MUC2, MMP7, and CK20 in CRC cell lines and different types of immune cells

From: Biomarker selection for detection of occult tumour cells in lymph nodes of colorectal cancer patients using real-time quantitative RT–PCR

  

Biomarker mRNA

Cells

Origin

CEA

CEACAM6

CEACAM1-S

CEACAM1-L

CEACAM7-1

CEACAM7-2

MUC2

MMP7

CK20

HT29

Colon iEC

32a

43

22

17

0.003

0.3

0.01

53

85

LS174T

Colon iEC

328

81

1.2

1.4

0.5

38

4.3

3.4

0.02

T84

Colon iEC

33

1.0

0.9

0b

0.003

0.07

0.5

2.3

33

HCT8

Colon iEC

32

0.5

0.003

0

0

0.1

0.02

0.2

0.05

PBMC

 

0

0.05

0

0.3

0.01

0

0

0.001

0

Act. PBMC

 

0

0

0.001

0.8

0

0

0

2.0

0

Jurkat

T cell

0

0

0.004

0.1

0

0

0

0.004

0

Molt-4

T cell

0

0

0.002

0.05

0

0

0

0

0

CNB6+KR4

B cell

0

0

0.008

1.1

0.05

0

0

0.6

0

U266

Plasma cell

0

0

0.001

0.02

0

0

0

0

0

U937

Monocyte

0.005

0.3

0.002

0.03

0

0

0

0.9

0.003

HL60

Granulocyte

0

1.8

0

ND

0

0.001

ND

0

0

K562

Pre-erythrocyte

0

0

0.006

0.08

0

0

0

0

ND

  1. aExpression level of the indicated mRNA species expressed as mRNA copies/18S rRNA unit.
  2. bValues below 0.001 mRNA copies/18S rRNA unit are recorded as 0.
  3. ND=not determined.
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