Table 1 Large and small intestinal apoptotic activity in p53 wild-type and p53 null mice following high dose 5-FU (200 mg kg−1)±NAC (200 mg kg−1)

From: Regional localisation of p53-independent apoptosis determines toxicity to 5-fluorouracil and pyrrolidinedithiocarbamate in the murine gut

  

Apoptotic index (%)

  

Large intestine

Small intestine

p53

Time (h)

5-FU

5-FU+NAC

5-FU

5-FU+NAC

Wild-type

Control

0.21

0.21

0.22

0.22

 

24

15.05

6.40 a

7.80

8.21

 

48

1.10

0.33

4.39

6.20 a

Null

Control

0.09

0.09

0.18

0.18

 

24

1.11

0.72

5.12

6.68

 

48

0.48

0.42

5.39

10.93 a

  1. Apoptotic indices are expressed for the crypt as a whole over cell positions 1–18. Six animals were used in each group, counting 50 half-crypts per mouse. Control values (saline injection) are shown in ‘normal typeface’ as are treatment indices that do not significantly differ from these baseline values (P>0.001, PC crypts). Treatment indices outside control values are displayed in bold (P<0.001, PC crypts).
  2. aA significant difference between 5-FU and 5-FU/NAC treatments at a specific time point (P<0.001, PC crypts).
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