Table 2 Cell cycle modulation after drug treatments, followed by 24-h culture in drug-free medium. Values (%) are means from three independent experiments and the differences (Δ) are calculated with respect to controls
Cell lines | Treatment | G1 phase | (ΔG1) | S-phase | Δ(S) | G2/M phase | Δ(G2/M) |
|---|---|---|---|---|---|---|---|
Control | 70.50 | 27.16 | 2.34 | ||||
Gemcitabine (1 h) | 49.20 | −21.30 | 46.57 | +19.41 | 4.32 | +1.98 | |
T24 | Pemetrexed (24 h) | 57.05 | −13.35 | 39.28 | +12.12 | 3.67 | +1.33 |
Gemcitabine-pemetrexed | 72.95 | +2.45 | 23.76 | −3.4 | 3.29 | +0.95 | |
Pemetrexed-Gemcitabine | 74.49 | +3.99 | 16.80 | −10.36 | 8.71 | +6.37 | |
Control | 44.32 | 39.46 | 16.22 | ||||
Gemcitabine (1 h) | 57.94 | +13.62 | 33.46 | −6.00 | 8.60 | −7.62 | |
J82 | Pemetrexed (24 h) | 37.95 | −6.37 | 51.34 | +11.88 | 10.71 | −5.51 |
Gemcitabine-pemetrexed | 28.90 | −15.42 | 61.20 | +21.74 | 9.90 | −6.32 | |
Pemetrexed-Gemcitabine | 24.49 | −19.83 | 61.12 | +21.66 | 14.38 | −1.84 |
