Table 1 Sequences of primer/probe sets used in real-time RT–PCR (TaqMan) analyses (5′ → 3′)
Accession no. | Forward/reverse primers | Probe | |
|---|---|---|---|
C/EBPα | AGAGCCGAGATAAAGCCAAACA CGGTCATTGTCACTGGTCAACT | AACGTGGAGACGCAACAGAAGGTGCT | |
C/EBPβ | ACAAGCTGAGCGACGAGTACAA TGTGCTGCGTCTCCAGGTT | CGCGAGCGCAACAACATCGC | |
PPARγ | AGTGGAGACCGCCCAGG GCAGCAGGTTGTCTTGGATGT | TTGCTGAACGTGAAGCCCATCGAG | |
Glut-4 | CATCCCACAAGGCACCCTC CATGCCACCCACAGAGAAGA | CTACGCTCTGGGCTCTCTCCGTGG | |
Leptin | CATCTGCTGGCCTTCTCCAA ATCCAGGCTCTCTGGCTTCTG | AGCTGCTCCCTGCCTCAGACCAGTG | |
SREBP-1c | GGCACTAAGTGCCCTCAACCT GCCACATAGATCTCTGCCAGTGT | TGCGCAGGAGATGCTATCTCCA | |
ACC | CCCAGCAGAATAAAGCTACTTTGG TCCTTTTGTGCAACTAGGAACGT | TGAGCATGGCATCCGGCGACT | |
FAS | CCTGGATAGCATTCCGAACCT AGCACATCTCGAAGGCTACACA | CCTGAGGGACCCTACCGCATAGC | |
SCD-1 | TCACGACCCCACCTATCAGG TTCCTCCAGACGTACTCCAGC | TGAGGAGGGACCCCCGCCC | |
GPAT | CAACACCATCCCCGACATC GTGACCTTCGATTATGCGATCA | TCGTCATACCCGTGGGCATCTCG | |
PGC-1α | GATGGCACGCAGCCCTAT CTCGACACGGAGAGTTAAAGGAA | CATTGTTCGATGTGTCGCCTTCTTGCT | |
UCP2 | AAAGATACTCTCCTGAAAGCCAACCT GGCCCCGAAGGCAGAA | ATGACAGATGACCTCCCTTGCCACTTCA | |
TNF-α | CCCAGACCCTCACACTCAGATC GCCACTCCAGCTGCTCCTC | TAGCCCACGTCGTAGCAAACCACCAAG | |
IL-6 | ACAACCACGGCCTTCCCTACTT CACGATTTCCCAGAGAACATGTG | TCACAGAGGATACCACTCCCAACAGACCT | |
MAC-1 | GAATGGATTGTGCTATTTGTTCGG CGGAGCCATCAATCAAGAAGAC | TCCAACCTGCTGAGGCCGCCC | |
F4/80 | AAGACTTGATACTCCAAAGTGAGC GAAGGAAGCATAACCAAGATCCC | CCCTGCACTGCTTGGCATTGCTGT | |
ZAG | GAGCCTGTGGGACCTTGGA CCTCCCTGGCCCTCTGAA | AATGGAGGACTGGGAGAAGGAAAGCCAG | |
β-Actin | ACGGCCAGGTCATCACTATTG CAAGAAGGAAGGCTGGAAAAGA | ACGAGCGGTTCCGATGCCCTG |
