Table 1 Markers studied, and conditions for immunohistochemistry
Antibody | Pretreatment | Dilution | Source of antibody |
|---|---|---|---|
Thymidylate synthase (TS) | 4 × 4 min microwave in citric acid with 10 min standing | 1/300 | Simon Joel, London, UK |
Cyclin A | 3 × 4 min microwave in citric acid with 10 min standing | 1/100 | #NCL-CYCLIN A, Novocastra Labs Ltd, UK |
Thymidine phosphorylase (TP) | None | 1/400 | #MS-499-P0, NeoMarkers Inc., USA |
Carbonic anhydrase-9 (CA-9) | None | 1/50 | Adrian Harris, Oxford, UK |
Glucose transporter-1 (GLUT-1) | 3 × 4 min microwave in citric acid with 10 min standing | I/200 | #A3536, DAKO Corporation, USA |
Hypoxia inducible factor-1α (HIF-1α) | 4 × 4 min microwave in citric acid with 20 min standing | 1/1000 | #ab463-100, Abcam Ltd, UK |
Vascular endothelial growth factor (VEGF) | 3 × 4 min microwave in Tris-HCl with 10 min standing | 1/100 | #MS-350-P1, NeoMarkers Inc., USA |
