Table 3 Spectral stain intensity and area limits for each visual grade (visual intensity score represents overall TS stain intensity, visual area score denotes the percentage of the section staining positively for TS)

From: The impact of surgically induced ischaemia on protein levels in patients undergoing rectal cancer surgery

Grade

1

2

3

4

Visual intensity score

Negative

Weak

Moderate

Strong

Spectral intensity range (a.u.)

0

0.53–0.66

0.75–0.88

0.91–1.11

Visual area score

<20%

20–50%

50–75%

>75%

Spectral area range (pixels × 105)

0.02–1.1

1.7–2.4

2.5–3.1

3.7–4.0

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