Figure 2
Tumorigenicity of Raji parental, PI and cured cells in SCID mice. (A) SCID mice were subcutaneously implanted with 1 × 107 cells of Raji parental, PI or cured cells. Tumour growth was followed for a period of 5 weeks and two-dimensional measurements were taken weekly with a caliper. Resected tumours were photographed. (B) Haematoxylin and eosin staining and IH of reovirus antigens in tumour tissue taken from Raji parental, PI and cured tumours. Haematoxylin- and eosin-stained section (original magnification × 200) show large tumours 30 days postinjection in the Raji and cured group. Tumours in the PI group were not apparent on palpation and only small foci of tumour were found on histological examination. Immunohistochemical of tumour sections (original magnification × 200) shows that only PI cells stain positively for reovirus proteins (brown), although most of the tumour is necrotic.
