Figure 3
Effect of reovirus on Raji parental and cured cells in vitro. (A) Cell viability. Raji parental, PI and cured cells were exposed to reovirus at an MOI of 20 PFU cell−1, and viability at 96 h postinfection was assessed using WST-1 assay from three independent wells. Compared to controls, P-values for reovirus-treated groups are P<0.001, P=0.93 and P=0.222 for Raji, Raji PI and Raji cured, respectively. (B) Viral protein synthesis. Infected cells were metabolically labelled with [35S]methionine for 6 h at 42 h postinfection. Cell lysates were prepared, and reovirus proteins were immunoprecipitated with a polyclonal anti-reovirus antibody and analysed by SDS–PAGE. The three size classes of reovirus proteins (λ, μ and σ) are indicated on the left. (C) Virus progeny. At 0 and 96 h postinfection, cells were harvested and freeze–thawed three times, and the virus titre in the lysate was determined by plaque assay on L929 cells. Compared to input virus, P-values for progeny virus at 96 h postinfection are P<0.001, P=0.0015 and P<0.001. Error bars indicate s.e.m. from three separate wells and *demonstrates statistical significance.
