Figure 2
From: Regulation of mammalian horizontal gene transfer by apoptotic DNA fragmentation
Inhibition of DNA fragmentation in donor cells led to decreased gene transfer. About 107 apoptotic control and mICAD-expressing donor cells containing puror gene were added to 2 × 106 p53−/− cells and co-cultured for 3 days. Then MEF cells underwent puromycin selection for 2 weeks and the frequency of puror gene transfer was calculated by counting the surviving colonies. About 107 apoptotic donor cells, 2 × 106 p53−/− MEF cells alone (without co-culture with apoptotic donors) were also plated in 10 cm dishes and underwent puromycin selection as controls. The donor cells are L929 (A) and TK6 (B) cells. (C) Confirmation of transferred gene in puromycin-resistant MEFs by PCR.
