Figure 3
From: Regulation of mammalian horizontal gene transfer by apoptotic DNA fragmentation
Lack of effect on HGT by CAD in the recipient cells. (A) Genotyping of CAD−/− mice and isolated MEF clones. Wild type produces a single upper band, heterozygote produces two bands, and the homozygous CAD knockout has a single lower band. (B) Transfer of puror gene to CAD−/− MEF cells. Parental TK6 or stable TK6 cells containing puror gene were induced to undergo apoptosis by radiation. The apoptotic cells were incubated with wild type, CAD−/−, or p53−/− MEFs for 3 days before puromycin selection.
