Figure 1
Foscan® localisation in MCF-7 cells. (A, C, E; left panel) Confocal overlay images and (B, D, F; right panel) fluorescence topographic profiles of MCF-7 cells double-stained with Foscan® and organelle probes. Arrow indicates the analysed longitudinal transcellular zone. Cells were sensitised with 1.5 × 10−6 M Foscani® for 24 h, washed and subjected to organelle staining. (A, B) Mitochondria were labeled with 500 × 10−9 M MitoTracker green for 30 min at 37°C. (C, D) ER was stained with 2.5 μg ml−1 DiOC6 for 1 min. (E, F) Golgi apparatus was labeled with 5 × 10−6 M BPC for 30 min at 4°C. Organelle-specific probes were excited with a 488-nm; Foscan® was excited at 633 nm. Objective magnification × 63.
