Figure 2
Kinetic of Foscan® localisation in Golgi apparatus in MCF-7 cells. Cells were sensitised with 1.5 × 10−6 M Foscan® from 3 to 24 h incubation time, washed and subjected to staining with 5 × 10−6 M BPC for 30 min at 4°C. Fluorescence topographic profiles of MCF-7 cells double-stained with Foscan® and BPC are presented under confocal overlay images of MCF-7 cells double-stained with BPC (arrow indicates the analysed longitudinal transcellular zone). Organelle-specific probes were excited at 488 nm; Foscan® was excited at 633 nm. Objective magnification × 63.
