Table 2 Foscan® concentrationa,b in whole cells and in mitochondria fraction with respect to the time of incubation with MCF-7 cells

From: Relationship between subcellular localisation of Foscan® and caspase activation in photosensitised MCF-7 cells

 

3 h

24 h

 

mol mg protein −1

mol mg protein −1

Total cell concentration

9.4 × 10−11±0.4

76.0 × 10−11±5.7

Mitochondria concentration

3.3 × 10−11±2.2

7.7 × 10−11±2.7

Relative contentc

33.6±3.7%c

10.2±1.2%c

  1. Foscan® concentration was assessed by fluorescence spectrometry from cell lysate.
  2. Mean±s.d. of at least triplicate determinations.
  3. cRelative content calculated as the percentage of Foscan® concentration in mitochondria to the intracellular Foscan® concentration.
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