Figure 5

P-glycoprotein function and expression affected by anti-Cripto Mab in CCRF-CEM and CEM-A7R tested by flow cytometry and Western blot. (A and C) Flow cytometry analysis of Rh123 accumulation in the absence (open histogram) or presence of 10 μ M Vp (solid histogram). (B) Rh123 accumulation in the CEM/A7R cells following 3 h treatment with 25 μg ml⁻¹ C13 in the absence (open histogram) or presence of 10 μ M Vp (solid histogram) or in the absence (open histogram) or presence of 25 μg ml⁻¹ C13 (solid histogram) (D). P-glycoprotein function is expressed as the ratio of MCF in the presence or absence of Vp or C13 as described in the ‘Materials and Methods’. P-glycoprotein levels were expressed as the ratio (R) of the MCF. (E) Western blot analysis of Pgp expression detected by Mab to human Pgp in the CEM/A7R cells following 6 h treatment with 10 μg ml⁻¹ C4, 0.02 μg ml⁻¹ AraC and combined use of the two reagents.