Figure 6 | British Journal of Cancer

Figure 6

From: Anti-Cripto Mab inhibit tumour growth and overcome MDR in a human leukaemia MDR cell line by inhibition of Akt and activation of JNK/SAPK and bad death pathways

Figure 6

Western blot showing signalling molecules mediated by C4, AraC and combination of the two reagents in the CEM/A7R cells. (A) Activation of JNK/SAPK examined by JNK kinase assay and blotted by anti- phosphor-c-Jun Mab in CEM/A7R cells treated with 10 μg ml−1 C4 and 0.02 μg ml−1 AraC or combination for 3 or 6 h. (B) Western blot analysis of phospho-Akt at Ser473 and cleavage of caspase 9 at Asp330 compared to the level of expression of Cripto and (C) Western blot analysis of phosphor-Bad at Ser136, Ser112, total Bad protein, phosphor-Bcl-2 at Ser70, Bcl-xL in the same cell lysates.

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