Figure 1 | British Journal of Cancer

Figure 1

From: Multiple mechanisms underlie defective recognition of melanoma cells cultured in three-dimensional architectures by antigen-specific cytotoxic T lymphocytes

Figure 1

Generation and characterisation of MCTS of melanoma cells. (A) In all, 5000 or 30 000 melanoma cells (NA8, HBL, and D10) were cultured on polyHEMA-coated plates to prevent cell attachment for 3 days, resulting in the formation of MTCS of an average diameter of 300–800 μm. (B) D10 cells proliferation was evaluated by alamarBlue™ Assay (Serotec, Oxford, UK). Divergent kinetics were detectable for D10 cells cultured in monolayer (2D) or MCTS (3D). Similar results were observed for NA8 (Ghosh et al, 2005b) and HBL cells (Ghosh et al, 2005a).

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