Figure 2 | British Journal of Cancer

Figure 2

From: Multiple mechanisms underlie defective recognition of melanoma cells cultured in three-dimensional architectures by antigen-specific cytotoxic T lymphocytes

Figure 2

‘Non-brisk’ infiltration of melanoma MCTS by iDC, total CD8+ T cells and antigen-specific CTL. NA8 and HBL cells were stained with PKH26 red fluorochrome and cultured on polyHEMA-coated plates for 3 days to form 30 000 cells MCTS. Immature DC, total CD8+ T cells from a healthy donor and a CTL clone specific for HLA-A*0201-restricted Melan-A/MART-127–35 epitope were labelled with CFSE. Cells were added at a 2.5 : 1 ratio to each MCTS and co-cultured for 24 h. Immature DC, total CD8+ T cells and TAA-specific CTL infiltration in NA8 and HBL MCTS, respectively, were analysed by confocal microscopy.

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