Figure 2 | British Journal of Cancer

Figure 2

From: Novel retinoic acid metabolism blocking agents have potent inhibitory activities on human breast cancer cells and tumour growth

Figure 2

The effects of RAMBA VN/14-1 on proliferation of three human breast cancer cell line. For MCF-7 and T47D cells, 24-well plates were used and for MDA-MB-231 cells, 96-well plates were used. Cells (10 000 cells per well of a 24-well plate and 1000 cells per well of a 96-well plate) were plated and allowed to attach for 24 h. Cells were treated with VN/14-1 dissolved in 95% ethanol on day 1 and day 4 and analysed on day 7 by MTT assay using the spectrophotometer (Victor 1420 multi-label counter, Wallac (Perkin Elmer, Waltham, MA, USA)). For each concentration of the drug there were triplicate wells (24-well plate) and six wells (96-well plate) in every individual experiment. The data presented are mean±s.e.m. for 2–3 experiments. IC50 values were calculated by nonlinear regression analysis using GraphPad Prism software.

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