Figure 4 | British Journal of Cancer

Figure 4

From: Novel retinoic acid metabolism blocking agents have potent inhibitory activities on human breast cancer cells and tumour growth

Figure 4

Western immunoblotting analysis of whole-cell lysates of treated MCF-7 cells for the expression of ER-α. MCF-7 cells were treated with ATRA and VN/14-1 for 6 days and then cell lysates were electrophoresed using 10% SDS–PAGE and subjected to Western blotting. Lane 1: control; lanes 2–4: ATRA (1, 5 and 10 μ M); and lanes 5–7: VN/14-1 (1, 5 and 10 μ M). Numbers below the blot show fold increase in expression of the protein as analysed by ImageQuant densitometry analysis. Membrane was stripped and probed for β-actin to verify equal protein loading. Primary antibody (Santa Cruz Biotechnology), 1 : 200 in 5% milk PBST for 2 h at RT, secondary antibody (anti-rabbit) 1 : 3000 in 5% milk PBST for 1 h at RT. The experiment was repeated twice with similar results.

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