Figure 6

Graphs showing apoptosis induced in (A) MCF-7 and (B) T47D cells as determined by TUNEL. (A) MCF-7 cells and (B) T47D cells (4 × 10⁴)/ well were plated in eight-well slide and treated with 5 μ M of ATRA, RAMBAs and 4-HPR for 6 days (see Materials and Methods for details). TUNEL-stained apoptotic cells were counted against the DAPI stained cells to obtain percentage apoptosis. The experiments were repeated thrice with similar results. Error bars show s.e.m of three different fields of treated cells, statistically significant ^*P<0.01 and ^**P<0.001 vs control.