Figure 2

(A) Results obtained from SQRT-PCR to estimate endogenous WTH3 gene expressions in 11 cell lines with defined p53-gene status. (B) Western blot analysis of WTH3 proteins in MCF7/AdrR vs MCF7/WT and MES-SA/D × 5 vs MES-SA using WTH3 (1 : 200 dilution) and Rab6 (1 : 2000 dilution) antibodies. In addition, MDR1 detected by the MDR1 antibody (1 : 30 dilution) served as one of the controls. Proteins and their molecular weights are indicated on the right and left. Identical SDS-PAGE gels with the same amount of protein (100 μg/lane) were loaded. Three gels were used for Western blot by three antibodies; the fourth gel was stained with Coomassie blue for protein concentration normalisation.