Figure 6
(A and B) results obtained from DAPI staining assays using Hela cells. In (A) and (B) Hela cells contain pcDNA3.1 and pcDNA/WTH3, respectively. Cells were stained with DAPI, and the stained nuclei were visualised under a fluorescent microscope (magnification, × 400). Arrows indicate apoptotic nuclear condensation. (C–F) results obtained from TUNEL assays using Hela and HEK293 cells as hosts. (C and D) HEK293 cells were transfected with pcDNA3.1 and pcDNA/WTH3, respectively, for 30 h. (E and F) Hela cells were transfected with pcDNA3.1 and pcDNA/WTH3, respectively, for 18 h. DNA fragmentation in the apoptotic cells induced by the WTH3 gene were stained with a brown colour as compare to the control cells containing the empty vector, which were blue in colour. (G–I) results generated by flow cytometry assays using HEK293 cells. (G) Cells served as vehicle control. (H and I) cells were transfected with pcDNA/3.1 and pcDNA/WTH3, respectively. The cells were then stained with PI and the intercellular fluorescence was measured by a flow cytometer. The sub-G1 DNA content in each group, which represented apoptotic cells, is indicated by a percentage.
