Figure 2

(A) Time course for neovascularisation in Matrigel plugs. C57BL/J6 mice were injected subcutaneously with 0.5 ml Matrigel with or without (control) bFGF. Twenty minutes before removing plugs from mice FITC-dextran (0.1 ml of a 0.25 mg ml⁻¹ solution) was injected i.v. to facilitate visualisation and quantification of angiogenesis. Plugs were removed on days 2–14 after injection (means±s.e.m., n=5, ^*P<0.05; ^***P<0.001 compared with corresponding controls). (B) Effect of 2-MeOE2 and 2-MeOE2bisMATE on Matrigel plug neovascularisation. Compounds were administered orally at the doses indicated for 7 days starting 24 h after Matrigel injection. On day 8 FITC-dextran was injected 20 min before removal of plugs from mice as described above. Representative photographs are shown from five mice per group. (C) Quantification of angiogenesis within the Matrigel plugs was achieved after FITC-dextran injection (means±s.e.m., n=5, ^*P<0.05, ^***P<0.001 compared with the level of vascularisation in Matrigel plugs supplemented with bFGF in animals receiving vehicle only, NS, nonsignificant).