Figure 3
From: In vivo inhibition of angiogenesis by sulphamoylated derivatives of 2-methoxyoestradiol
(A) The effect of dosing with 2-MeOE2bisMATE (50 mg kg−1, p.o.) for 1–4 days on neovascularisation of Matrigel plugs. After dosing for 1–4 days plugs were removed on day 7 after Matrigel injection. FITC-dextran was used as described previously to facilitate visualisation and quantification of angiogenesis. Representative photographs of plugs from groups of five animals are shown. (B) Quantification of angiogenesis within Matrigel plugs shown in (A) after FITC-dextran injection (means±s.e.m., n=5, ***P<0.001 compared with bFGF). (C) The effect of dosing with 2-MeOE2bisMATE (50 mg kg−1, p.o.) on established Matrigel plug vasculature. Neovascularisation was allowed to develop for 7 days before animals were dosed for 1 day or daily for 3 days. Plugs were removed at end of day 14 for photography and quantification after FITC-dextran injection. (D) Quantification of angiogenesis within Matrigel plugs shown in Figure 3c after FITC-dextran injection (means±s.e.m., n=5, *P<0.05 compared with bFGF; NS, nonsignificant).
