Figure 5
From: In vivo inhibition of angiogenesis by sulphamoylated derivatives of 2-methoxyoestradiol
(A) Inhibition of growth of tumours derived from MCF-7 cells in nude mice. Animals were dosed orally with 2-MeOE2bisMATE or 17-Cym-2-MeOE2MATE (5 mg kg−1 or 20 mg kg−1) or 2-MeOE2 (20 mg kg−1) 5 days per week for 3 weeks. Tumour volumes were measured weekly (means±s.e.m., n=5-10, ***P<0.001 compared with controls; NS, nonsignificant). (B) Section of tumours were prepared and stained for VEGFR-2 (× 200 magnification; scale bar, 1 cm=200 μm)) as indicated by the arrows. (C) Quantification of staining for VEGFR-2 revealed that at 20 mg kg−1 both 2-MeOE2bisMATE and 17-Cym-2-MeOE2MATE significantly reduced the expression of this receptor (means±s.e.m., n=3, *P<0.05, NS, nonsignificant). (D) Section of tumours were prepared and stained for Ki67, a marker of cell proliferation (× 200 magnification). (E) Quantification revealed that at both doses tested a significant reduction in Ki67 expression occurred (means±s.e.m., n=3, **P<0.01, ***P<0.001 compared with control).
