Figure 1
From: Heparan sulphate synthetic and editing enzymes in ovarian cancer
(A) HS6ST1 ISH shows RNA to be present in ovary tumour and endothelial cells, but absent in stroma and normal ovary (inset). (B) HS6ST2 ISH shows RNA to be present in ovary tumour cells, but absent in endothelial cells, stroma and normal ovary (inset). (C) 10E4, an anti-HS monoclonal antibody was used to demonstrate that intact HS chains were only present on endothelial cells in ovarian tumours. (D) 3G10 antibody was used to detect the HS stubs that remain after heparinase digestion. A negative control shows 3G10 staining to be negative without prior heparinase digestion (inset). (E) Heparanase ISH shows RNA to be present in ovary tumour cells, but absent in endothelial cells and stroma. (F) Heparanase IHC shows protein to be present in ovary tumour cells, but absent in endothelial cells and stroma. (G) Heparanase IHC on normal human ovary shows protein to be present at a low level in most cells. (H) HSULF-1 ISH shows RNA to be present in ovary tumour cells, but absent in endothelial cells, stroma and normal ovary (inset). Each scale bar represents 400 μm.
