Figure 3
From: Expression of VEGFxxxb, the inhibitory isoforms of VEGF, in malignant melanoma
Distal and proximal splice variants of VEGF are found in archival melanoma tissues. (A) Extraction of mRNA from 8- to 10-year-old archival sections of melanoma and surrounding skin followed by RT–PCR using primers in exon 7 and the 3′ UTR of the VEGF gene resulted in two products of 150 and 200 bp. These were consistent with VEGF165b and VEGF165, respectively. (B) Splice site-specific primers to the proximal splice site (PSS) and the distal splice site (DSS) confirmed expression of these isoforms in archival sections.
