Figure 3
Membrane localisation and effect of DESC1 expression on MDCK cells motility. (A) Immunocytochemical detection of recombinant DESC1 expression in MDCK cells. The images were captured by fluorescence microscopy of MDCK cells transfected with pcDNA-HA-DESC1 vector or with the same pcDNA3-HA plasmid containing the cDNA for polyserase-1. Immunofluorescent detection of anti-HA antibodies was carried out with a fluorescein-conjugated anti-mouse antibody, and detection of anti-DESC1 antibodies with a Texas Red-conjugated anti-rabbit antibody. Result shows the membrane localisation of DESC1. (B) Wound closure assay. Scrape wounds were made in confluent monolayers of cells stably transfected with vector containing DESC1 or control vector. Cell layers were imaged at the indicated times. (C) Matrigel invasion assay. Invasion capacity of MDCK/DESC1 cells and control transfectants were analysed by a Matrigel invasion assay after 48 h of incubation. Number of occluded pores by the cells and by the control cells transfected were counted and represented on the right (*P<0.0001). N indicates the number of microscopic fields analysed.
