Figure 5
DESC1 expression in kidney, liver, brain and breast normal and tumour tissues. (A) (a) Immunostaining of the epithelial component of a larynx tissue sample using the antibody generated in this work. (b) Immunostaining performed with a preimmune control serum does not show this pattern. (B) (a–d) IHC in kidney. (a) Normal kidney showing DESC1 expression in tubules (arrow). (b) Reactive tubules show positive expression (arrow), the proximal inflammated and atrophical region is negative (arrowhead in b and c), and the derived well-differentiated clear cell tumour in (c) is negative (see arrow). (d) Sample from a tissue array slide showing positivity in a poorly differentiated renal clear cell tumour. Note the negativity in the stroma. (e–f) IHC in liver. (e) Liver tissue showing intense signal in the pericentral hepatocytes. (f) Renal invasive hepatocarcinoma corresponding to the same specimen as in (e). Staining diminishes in the nodal satellite (arrow) that compresses some positive normal hepatocytes (arrowhead). (g–h) IHC of the brain. (g) Leptomeninges (dura mater) showing negative expression of DESC1. (h) Atypical meningioma showing positivity. (i–l) IHC of breast. (i) Reactive benign breast tissue showing slight positivity surrounding a negative duct. (j) Negative immunostaining in a ductal micropapilar breast tumour. (k) Positivity in a ductal breast carcinoma. (l) Positivity in a lobular breast carcinoma. Magnification was 20 × and counterstaining was with haematoxylin.
