Figure 1
From: MT1-MMP is the critical determinant of matrix degradation and invasion by ovarian cancer cells
Membrane-type 1-MMP expression in ovarian cancer cell lines. (A) Real-time PCR results for MT1 expression by ovarian cancer cell lines grown on a collagen I film. Results of RT–PCR for MT1 are depicted below, with amplification of the endogenous control gene RPL13a shown to confirm the use of equal template in all reactions. (B) Western blot with polyclonal antibody Ab815 showing detection of MT1 expression in lysates of cells grown on a collagen I film. The blot was re-probed for β-tubulin to verify equal loading. (C) Gelatin zymography for pro-MMP-2 activation by cells grown on a 3D collagen I gel and incubated with HGF-conditioned media as an exogenous source of pro-MMP-2. Levels of pro-MMP-2 provided in the HGF-conditioned media are shown in the CTRL lane (in absence of cells).
