Figure 2
From: MT1-MMP is the critical determinant of matrix degradation and invasion by ovarian cancer cells
Collagen I transwell invasion, matrix degradation and motility of ovarian cancer cell lines. (A) Collagen I transwell invasion capacity. Results from three independent experiments were normalised to HEY and data were pooled. Bars represent the mean±s.e. (nd – HEY invasion was not assessed beyond 24 h as invasion was complete by this time). (B) Clearance/degradation of collagen I plugs from transwell invasion membranes. Confocal fluorescence microscopy was used (× 16) to visualise the biotinylated collagen. Representative areas near the centre of the membrane are shown and arrows highlight transwell pores that have been cleared of their collagen I plugs, except for HEY, in which all pores were cleared. Inset shows an enlarged image that distinguishes cleared pores (black) from uncleared pores (grey). (C) Transwell migration through unpolymerised collagen I-coated transwells at 8–10 h. Results from three independent experiments were normalised to HEY and data were pooled. Bars represent the mean±s.e.
