Figure 4
From: MT1-MMP is the critical determinant of matrix degradation and invasion by ovarian cancer cells
MT1 enhances invasion solely through its promotion of matrix degradation; not by affecting cell motility. (A) Degradation of biotinylated collagen I matrices by MT1-transfected SKOV-3 and OVCAR-3 cells after a 24 h incubation. Matrices were not degraded by cells expressing the E240A catalytically inactive MT1 mutant, nor by control untransfected (UT) cells. Cells were visualised via rhodamine phalloidin staining of actin (red) and biotinylated collagen matrices were visualised using streptavidin AF488 (green), using confocal microscopy (× 40). (B) Overexpression of MT1 or E240A did not affect cell motility on collagen. Cells were seeded in transwell chambers pre-coated with 20 μg unpolymerised collagen and allowed to migrate for 8–10 h. Results shown are the mean±s.e. for three independent experiments.
