Figure 4
(A) Cytotoxicity of sapacitabine in a panel of 10 cancer cell lines. (B) Profiling mRNA levels of metabolic factors in 10 cell lines. Analysis of main nucleoside metabolism factors (hENT1, dCK, DNA polymerase and cN-II) was performed by quantitative real-time PCR. RNA was extracted from cells to determine transcript copy numbers relative to the reference gene 18S. Results were expressed as PCR arbitrary units in all cell lines related to HL-60 cells PCR arbitrary unit expression.
