Figure 2 | British Journal of Cancer

Figure 2

From: PLD1 is overexpressed in an ER-negative MCF-7 cell line variant and a subset of phospho-Akt-negative breast carcinomas

Figure 2

Phospholipase D1 (PLD1) mRNA levels are highly expressed in basal-like breast carcinomas. Total RNA was isolated from frozen human–breast tumour specimens, and gene expression was determined using real-time RT–PCR. An arbitrary cutoff point was used to select tumours that expressed high levels of cytokeratin (CK5), CK17, and PLD1. Phospholipase D1 mRNA levels were significantly higher in tumours that expressed high levels of CK5 and/or 17 (P<0.01). Phospholipase D1 mRNA levels were not significantly higher in oestrogen receptor (ER)-negative tumours (1–18 are ER positive, and 19–30 are ER negative, P>0.05, see text for details). Samples were tested two or three times; s.d. represents variation between PCR experiments.

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