Figure 1

Effect of 1 and 20 nM paclitaxel on microtubular network and chromosome segregation in untransformed human mammary epithelial cells. (A) Immunostaining of α-tubulin (green) in untreated (CTRL), 1 and 20 nM paclitaxel-treated (PTX 1 and 20 nM) HME1 cells. White arrows indicate microtubule bundles. Scale bar, 10 μm. (B) Micronucleus detection in paclitaxel-treated PSP and CC cells by CBMN assay. Briefly, 10⁶ cells ml⁻¹ were seeded 24 h before paclitaxel (PTX) treatment (24 h). Cytochalasin-B (CytoB) at 5 μg ml⁻¹ was added in culture media 6 h after the beginning of paclitaxel exposure (6 h). After 30 h of paclitaxel treatment, cells were fixed by methanol, DNA was stained with Hoechst 33258 and binucleated cells with micronuclei (MN) were scored. White arrows indicate micronuclei in binucleated cytokinesis-blocked HME1 cells treated by 1 nM paclitaxel. Scale bar, 10 μm. CBMN, cytokinesis-block micronucleus.