Figure 1

VEGF protein expression in gastric cancer tissue. Representative microphotographs of negative (A–C), weak (D–F) and high expression (G–I) of VEGF. Immunohistochemical staining for VEGF was performed using a specific antibody against VEGF. (A, D, G) H&E staining. (B, E, H) Immunostaining for VEGF. (C, F, I) Representative areas from (B) (E) and (H), respectively. The downstaged cases showed a significantly higher expression of VEGF than non-downstaged cases by Mann–Whitney U-test (P=0.043) (J).