Table 3 Results of annexin V-PE flow cytometry upon 72 h exposure to chemotherapy

From: [18F]2-Fluoro-2-deoxy-D-glucose incorporation by AGS gastric adenocarcinoma cells in vitro during response to epirubicin, cisplatin and 5-fluorouracil

  

Cells, % (±s.e.m.)

Chemotherapy

Dose

Annexin V-PE and 7-AAD negative

Annexin V-PE positive and 7-AAD negative

Annexin V-PE and 7-AAD positive

Control

 

93 (1)

4 (1)

2 (0)

Epirubicin

LD10

94 (1)

2 (1)

4 (0)

 

LD50

23 (1)

39 (1)

38 (0)

Cisplatin

LD10

95 (0)

3 (0)

2 (0)

 

LD50

80 (2)

15 (2)

6 (0)

5-Fluorouracil

LD10

89 (0)

7 (0)

4 (0)

 

LD50

93 (2)

3 (1)

4 (2)

  1. Annexin V-PE FACS, 10 000 events counted per sample with results displayed as a percentage of valid counts (annexin V-PE and 7-AAD negative=healthy cells; annexin V-PE positive and 7-AAD negative=early apoptosis; annexin V-PE and 7-AAD positive=necrosis or late apoptosis).
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