Figure 2

Time course of expression of thymidylate synthase (TS) and cyclin E in synchronised HCT116 cells. (A) Time-dependent changes in cyclin E and TS mRNA. Cyclin E and TS mRNA levels were quantified by real-time PCR and normalised to levels of RPL32 mRNA. Data from three independent experiments are shown. Error bars represent the s.e.m. (B) Western blot analysis of cyclin E and TS. (C) Relative expression of cyclin E and TS protein levels as determined by densitometry of blots shown in (B). Expression levels were normalised to 0 time and to β-actin levels.