Figure 4
Effect of adenovirus-mediated over-expression of E2F1, Dp1 and cyclin E on thymidylate synthase (TS) expression. (A) Western blot analysis of HCT116 cells infected for 24 h at multiplicity of infections (MOIs) of 10 and 25. (B) Relative mRNA expression levels of TS, cyclin E and E2F1 24 h after infection with adenoviruses encoding E2F1, Dp1 and cyclin E. mRNA levels were normalised to β-2-microglobulin mRNA levels. The mean and s.e.m. of three independent experiments is shown. (C) Western blot analysis of MCF-7 cells infected for 24 h at MOIs of 10 and 25. (D) Western blot analysis of GM38 cells infected for 24 h at a MOI of 25. Infection with CA35 adenovirus was used as a control in all panels.
