Figure 5
Effect of small molecule inhibitors of cyclin-dependent kinases (CDKs) on HCT116, MCF-7 and PC3 cells. Western blot analysis of thymidylate synthase (TS), cyclin E and cyclin D1 following 24 h treatment with solvent (dimethyl sulphoxide (DMSO)), roscovitine, CDK2 inhibitor or CDK4 inhibitor of HCT116 (A), MCF-7 (C) and PC3 (D) cells. (B) Cell-cycle distribution in HCT116 cells following 24 h treatment with the indicated inhibitor. Cell-cycle distribution was determined by flow cytometry as in Figure 1. Percentage of cells in G0/G1 is represented by the speckled bar, S-phase by the black bar and G2/M by the white. Data shown are the average of two independent experiments.
